The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。
FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。
Fe3 Reduces the Titer of bet-Deficient FFV and Induces Genome Editing. The effect of fe3 coexpression with WT and bet -deficient FFV genomes was studied after transfection of。
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